🧠 Pystackreg Web Application

📘 Credits & Acknowledgments

Register TIFF stacks using multiple alignment strategies.

App Author: Quentin Chappuis
Pystackreg Author: Gregor Lichtenberg
🔗 Pystackreg on GitHub

Original Algorithm Author: Philippe Thévenaz (EPFL)
The core algorithm was originally developed by Philippe Thévenaz and is described in the following publication:

P. Thévenaz, U.E. Ruttimann, M. Unser
A Pyramid Approach to Subpixel Registration Based on Intensity
IEEE Transactions on Image Processing, vol. 7, no. 1, pp. 27–41, January 1998.
🔗 View paper

Overview

This app provides three registration modes for 2D TIFF image stacks using the pystackreg library.

📚 Tab 1: Reference-Based Alignment

Register a stack using a reference frame. You can:

  • Use a frame from the same stack as reference
  • Or upload an external reference stack
  1. Upload the stack you want to align.
  2. (Optional) Check "Use external reference stack" to align to a frame from another file.
  3. Choose the reference frame using the slider.
  4. (Optional) Choose transformation mode.
  5. Click ▶️ Align Stack.
  6. Use sliders to browse original/aligned results and download the output.

🎯 Tab 2: Stack-Based Alignment

Align one stack (moving) to another (reference).

  1. Upload both reference and moving stacks.
  2. (Optional) Choose transformation mode.
  3. Click ▶️ Register to align.
  4. Browse and download registered stack.

🧩 Tab 3: Frame-to-Frame Alignment

Align a single frame to another from the same stack.

  1. Upload a stack.
  2. Select reference and moving frames using sliders.
  3. Choose transformation mode.
  4. Click ▶️ Register Frame.
  5. View/download the result.

🔄 Reset Buttons

Each tab includes a Reset Tab button that clears inputs, outputs, and internal state.

🧠 Credits

App developed by Quentin Chapuis
Library: pystackreg by Georg Lichtenberg
Try with Example TIFF
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